Preface

Chapter 1: Cell line development

Hansjoerg Hauser

Chapter 2: Transient recombinant protein expression in mammalian cells

Volker Jäger, Konrad Büssow, Thomas Schirrmann

Chapter 3: Hybridoma technology for the in vitro production of therapeutic antibodies

Susan McDonnell

Chapter 4: Bioreactors for mammalian cells

R. Pörtner

Chapter 5: Mass Transfer and Mixing Across the Scales in Animal Cell Culture

Alvin W Nienow

Chapter 6: Hydrodynamic Damage to Animal Cells

Jeffrey J Chalmers and Ningning Ma

Chapter 7: Monitoring of cell culture

Sebastian Schwamb, Robert Puskeiler, Philipp Wiedemann

Chapter 8: Serum and protein free media

Michael Butler

Chapter 9: Glycosylation in Cell Culture

Maureen Spearman and Michael Butler

Chapter 10: Modelling of mammalian cell cultures

Nishikant P. Shirsat, Niall J. English, Brian Glennon and Mohamed Al-Rubeai

Chapter 11: Mammalian Cell Line Selection Strategies for High-Producers

Darrin Kuystermans and Mohamed Al-Rubeai

Chapter 12: Building a Cell Culture Process with Stable Foundations: Searching for Certainty in an Uncertain World

Peter M O'Callaghan and Andrew J Racher

Chapter 13: Perfusion Processes

Veronique Chotteau

Chapter 14: Single-use bioreactors for animal and human cells

Stephan C. Kaiser, Matthias Kraume, Dieter Eibl, and Regine Eibl

Chapter 15: An Overview of Cell Culture Engineering for the Insect Cell-Baculovirus Expression Vector System (BEVS)

Laura A. Palomares, Mauricio Realpe and Octavio T. Ramirez

Chapter 16: Metabolic flux analysis: a powerful tool in animal cell culture

João V. Sá, Tiago M. Duarte, Manuel J. T. Carrondo, Paula M. Alves, Ana P. Teixeira

Chapter 17: Cell immobilization for the production of viral vaccines

Ana Carina Silva, António Roldão, Ana Teixeira, Paulo Fernandes, Marcos F.Q. Sousa, Paula M. Alves

Chapter 18: Cell Engineering for Therapeutic Protein Production

Eric Baek, Che Lin Kim, Jin Hyoung Park, and Gyun Min Lee

Chapter 19: Proteomics in Cell Culture: From genomics to combined 'omics for cell line engineering and bioprocess development

Kelley Heffner, Christian Kaas, Amit Kumar, Deniz Baycin-Hizal, Michael Betenbaugh

Chapter 20: Metabolomics in animal cell culture

Miroslava Čuperlovic-Culf

Chapter 21: Process Analytical Technology and Quality-by-Design for Animal Cell Culture

Stephen Craven and Jessica Whelan

Chapter 22: Biosafety recommendations on the handling of animal cell cultures

Philippe Herman and Katia Pauwels

Chapter 23: Biopharmaceutical products from animal cell culture

Darrin Kuystermans and Mohamed Al-Rubeai

Index