This volume covers the latest advancements and techniques used to understand the fastidious bacterium, Borrelia burgdorferi, and its significance in infectious disorders by combining both conventional and cutting-edge approaches. This book covers diverse topics, including direct detection, diagnostic methods, immune response analysis, alternative model systems, advanced proteomics, social media analysis, and clinical research. It also discusses unconventional wet lab research such as content analysis, the use of ChatGPT, clinical algorithms for chronic Lyme, establishment of a pregnancy Lyme disease biobank, and investigates Lyme in pregnant women. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Borrelia burgdorferi: Methods and Protocols encompasses a wide range of techniques and caters to scientists from various disciplines and career stages, such as cell and molecular biologists, statisticians, and clinical researchers.