9 11/16 X 7 7/16 in Contributors
Preface to the 2nd edition
Prefac e to the 1st edition
Introduction
The immune response to infectious agents
General Methods
Phenotyping and separation of leukocyte populations based on affinity labelling.
Cytometric cytokine secretion assay: detection and isolation of cytokine secreting T cells.
Measurement of cellular prol iferation.
Use of bioinformatics to predict MHC ligands and T-cell epitopes: application to epitope driven vaccine design.
The use of tetramers in the quantitative analysis of T cell respons es.
Using microarrays for studying the host transcriptional response to microbial infection.
Genome-wide expression pr ofiling of intracelllular bacteria: the interaction of Mycobacterium tub erculosis with macrophages.
Murine Mo dels
Management of immuno-compromised and infected animals.
In Vitro Analysis
Isolation
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