This book constitutes the refereed proceedings of the 16th IFIP WG 8.1 International Conference on Informatics and Semiotics in Organisations, ICISO 2015, held in Toulouse, France, in March 2015. The 21 revised papers presented were carefully reviewed and selected from 46 submissions. The papers are organized in the following topical sections: organisational semiotics: theory and concepts; organisational semiotics and applications; information systems and services; complex system modeling and simulation; and innovation and organisational learning.