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PCR Mutation Detection Protocols

PCR Mutation Detection Protocols

by Bimal D.M. Theophilus and Ralph Rapley
Hardback
Publication Date: 26/10/2010

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Since the publication of the popular first edition, the explosion of DNA sequence information, the access to bioinformatics and mutation databases coupled with the ability to readily detect and confirm mutations has cemented the role of molecular diagnostics in medicine and, in particular, mutation detection by the polymerase chain reaction (PCR). In PCR Mutation Detection Protocols, Second Edition, expert researchers bring the subject up-to-date with key protocols involving the PCR and its many various incarnations such as SSCP, CSGE, and dHPLC. The volume also addresses key areas such as Southern blotting, accurate diagnostics with high throughput, as well as microarray systems. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include brief introductions their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes which provide the often hard to find information that may mean the difference between the success and failure of the method.

Authoritative and cutting-edge, PCR Mutation Detection Protocols, Second Edition aims to stimulate postgraduate scientists, researchers, and clinicians already engaged in the area and to provide an important first step for those new to this practice wanting to adopt the powerful and essential technique in their own laboratories.
ISBN:
9781607619468
9781607619468
Category:
Medical genetics
Format:
Hardback
Publication Date:
26-10-2010
Language:
English
Publisher:
Humana Press Inc.
Country of origin:
United States
Edition:
2nd Edition
Pages:
298
Dimensions (mm):
254x178x19mm
Weight:
1.66kg

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