Table of Contents

Preface for Somatic Genome Manipulation: Advances, Methods and Applications

Xiu-Qing Li, Ph.D., Research Scientist of Molecular Genetics, Agriculture and Agri-Food Canada, xiu-qing.li@agr.gc.ca; somaticgenome@gmail.com.

Danielle Donnelly, Ph.D., Associate Professor, Plant Science Dept., McGill University, danielle.donnelly@mcgill.ca.

Thomas G. Jensen, M.D., D.M.Sc., Professor of Medical Genetics and Head of the Department of Biomedicine, Aarhus University, Denmark, thomas@hum-gen.au.dk.

Section I. Humans and animals

Chapter 1. Drug and Gene Electrotransfer in Cancer Therapy. (Dr. Julie Gehl, M.D., Dr. Med. Sci., Consultant Oncology

C*EDGE (Center for Experimental Drug and Gene Electrotransfer), Department of Oncology, Copenhagen University Hospital Herlev, Herlev Ringvej 75, 2730 Herlev, Denmark, E-mail: Julie.Gehl@regionh.dk.

Chapter 2. Targeted Porcine Genome Engineering with TALENs. Yonglun Luo ^{*, 1}; Lin Lin ¹; Monika M. Golas ^{1, 2}; Charlotte Brandt S rensen ^{1, 3}; and Lars Bolund ¹;

¹Department of Biomedicine, Aarhus University, Aarhus C, 8000, Denmark, Yonglun ALUN@HUM-GEN.AU.DK

²Center for Stochastic Geometry and Advanced Bioimaging, Aarhus University, Aarhus C, 8000, Denmark

³Department of Clinical Medicine - The Department of Cardiological Medicine B, Aarhus University, Brendstrupgårdsvej 100, 8200 Aarhus N, Danmark

Chapter 3. Somatic Gene Therapy using Viral Vectors: theoretical and clinical implications in relation to treatment of genetic conditions in humans. Anne Louise Askou and Thomas J. Corydon* Department of Biomedicine, Faculty of Health, Aarhus University, Denmark *Corresponding author: TJC, Department of Biomedicine, Faculty of Health, Wilhelm Meyers Allé 4, Aarhus University, 8000 Aarhus C, Denmark, Phone: (+45) 28992179; E-mail: tjc@hum-gen.au.dk.

Chapter 4. Nonviral Gene Therapy - The Challenge of Mobilizing DNA. Jacob Giehm Mikkelsen* MD, *Corresponding author. Department of Biomedicine, Aarhus University, Wilh. Meyers Allé 4, DK-8000 Aarhus C, Denmark. Phone: +45 87167767. Fax: +45 86123173. Email: giehm@hum-gen.au.dk.

Chapter 5. Human Stromal Stem Cell Therapy Using Gene Modified Cells

Walid Zaher^{1, 3} and Moustapha Kassem^{1, 2, 3}: ¹Department of Endocrinology and Metabolism, Endocrine Research Laboratory (KMEB), Odense University Hospital & University of Southern Denmark, Odense, Denmark. ²Danish Stem Cell Center (DanStem), Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark. ³Stem Cell Unit, Department of Anatomy, College of Medicine, King Saud University, Saudi Arabia.

* Corresponding author. Moustapha Kassem, professor, Endocrine Research Laboratory (KMEB), Department of Endocrinology, Odense University Hospital, Kloevervaenget 6, 4th floor, DK-5000 Odense C, Denmark. Tel: 45-65504084, 45-65411606; Fax: +66503920; E-mail: mkassem@health.sdu.dk

Chapter 6. Somatic Cell Nuclear Transfer and the Creation of Transgenic Large Animal Models. Naomi Dicks, Luis B. Agellon* and Vilceu Bordignon*, *Corresponding authors. Mailing address:

Vilceu Bordignon, McGill University, Department of Animal Science, 21,111 Lakeshore Road, Ste-Anne-de-Bellevue, Quebec, H9X 3V9, Canada. Phone: 514-398-7793. Fax: 514-398-7964. E-mail: vilceu.bordignon@mcgill.ca

Luis B. Agellon, McGill University, School of Dietetics and Human Nutrition, 21,111 Lakeshore Road, Ste-Anne-de-Bellevue, Quebec, H9X 3V9, Canada. Phone: 514-398-7862. Fax: 514-398-7739. E-mail: luis.agellon@mcgill.ca

Section II. Plants