After centuries of sinful bloodshed, Nicholas Knight has forsaken immorality and is determined to reclaim his humanity. He now spends his nights as a homicide detective with his smug partner, Donald Schanke, fighting the city's most brutal crimes. Joining Nicholas' quest is Dr Natalie Lambert, a brilliant forensic scientist, who is inching closer to the cure for his supernatural condition. The master vampire Lucien LaCroix however, will stop at nothing to ensure that Nicholas remains a vampire. In his darkest moments, Nicholas turns to the lovely vampire Janette, who offers him unconditional loyalty despite her prominent standing in the vampire underworld.
As the stakes get higher and his path to redemption becomes burdened with temptations, Nicholas valiantly sets out to conquer his curse night be night.