The book explores the convergence of AI and Digital Twin technologies to empower Smart Manufacturing initiatives. Through a comprehensive examination of principles, methodologies, and practical applications, the book explains the transformative potential of AI-enabled Digital Twins across various facets of the manufacturing lifecycle. From design and prototyping to production and maintenance, AI-enabled Digital Twins offer multifaceted advantages that redefine traditional paradigms. By leveraging AI algorithms for data analysis, predictive modeling, and autonomous optimization, manufacturers can achieve unparalleled levels of efficiency, quality, and agility. The book demonstrates how the promise to enhance competitiveness, reduce operational costs, and facilitate innovation in the manufacturing industry is possible. By harnessing AI's capabilities in conjunction with digital twins, manufacturers can achieve a more agile and responsive production environment, ultimately driving the evolution of smart factories and Industry 4.0/5.0.