This book explores the medical importance of Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter spp. (ESKAPE) pathogens, and the challenges posed by antibiotic resistance. It covers the antibiotic resistance profile and detection of ESKAPE pathogens, as well as the mechanistic understanding of antibiotic resistance in these pathogens. The book discusses standard microbiological techniques, including staining, morphological and cultural characteristics, biochemical properties, and serotyping, as well as nucleic acid amplification and molecular diagnostic techniques for the detection of ESKAPE pathogens. In addition, the book explores biochemical, molecular, and computational techniques for the determination of virulence factors of ESKAPE pathogens. It also examines the synthesis, structure, function, and inhibitors of virulence factors and biofilm componentsin these pathogens. The book explores various strategies for combating ESKAPE pathogens, including antibiotic adjuvants and their synergistic activity, phytochemicals as potential antibacterial agents, photodynamic therapy, antimicrobial peptides and antibacterial antibodies, antimicrobial activity of Nanomaterials and Nanocomposites, and bacteriophage therapy. Finally, the book discusses in silico and computational approaches for the inhibition of ESKAPE pathogens. This comprehensive book is of interest to researchers, students, and healthcare professionals interested in the diagnosis, prevention, and treatment of ESKAPE pathogens.