Part I: Detection, Quantification, and Confirmation

1. Traditional Methods of Analysis for Listeria monocytogenes

Rui Magalhães, Cristina Mena, Vânia Ferreira, Gonçalo Almeida, Joana Silva, and Paula Teixeira

2. MALDI-ToF MS: A Rapid Methodology for Identifying and Subtyping Listeria monocytogenes

Snehal R. Jadhav, Rohan M. Shah, and Enzo A. Palombo

3. Sample Preparation for qPCR Detection of Listeria from Food

Patrick Mester, Anna Kristina Witte, and Peter Rossmanith

4. qPCR Validation on the Basis of the Listeria monocytogenes prfA Assay

Anna Kristina Witte, Patrick Mester, and Peter Rossmanith

Part II: Subtyping Approaches

5. Serotype Assignment by Sero-Agglutination, ELISA, and PCR

Lisa Gorski

6. Pulsed-Field Gel Electrophoresis (PFGE) Analysis of Listeria monocytogenes

Karen Hunt and Kieran Jordan

7. Multi-Locus Sequence Typing (MLST) and Whole Genome Sequencing (WGS) of Listeria monocytogenes and Listeria innocua

Beatrix Stessl, Martin Wagner, and Werner Ruppitsch

Part III: Genotypic and Phenotypic Characterization

8. High-Throughput Characterization of Listeria monocytogenes Using the OmniLog Phenotypic Microarray

Laura Luque-Sastre, Kieran Jordan, Séamus Fanning, and Edward M. Fox

9. High-Throughput Screening of Biofilm Formation of Listeria monocytogenes on Stainless Steel Coupons Using a 96-Well Plate Format

Jessica A. Gray, P. Scott Chandry, John P. Bowman, and Edward M. Fox

10. Confocal Laser Microscopy Analysis of Listeria monocytogenes Biofilms and Spatially Organized Communities

Maud Darsonval, Marina Grégoire, Julien Deschamps, and Romain Briandet

11. Extraction and Preparation of Listeria monocytogenes Subproteomes for Mass Spectrometry Analysis

Tiago Santos and Michel Hébraud

Part IV: Strain Manipulation

12. Extraction and Analysis of Plasmid DNA from Listeria monocytogenes

Aidan Casey and Olivia McAuliffe

13. Generation of Nonpolar Deletion Mutants in Listeria monocytogenes Using the "SOEing" Method

Kathrin Rychli, Eva Harter, Caitriona M. Guinane, Karen Daly, Colin Hill, and Paul D. Cotter

14. Mutant Construction and Integration Vector-Mediated Genetic Complementation in Listeria monocytogenes

Cameron Parsons, Reha Azizoglu, Driss Elhanafi, and Sophia Kathariou