1. Recreating the Follicular Environment: A Customized Approach for In Vitro Culture of Bovine Oocytes Based on the Origin and Differentiation State

Alberto Maria Luciano, Rodrigo Garcia Barros, Ana Caroline Silva Soares, Jose Buratini, Valentina Lodde, and Federica Franciosi

2. Clinostat 3D Cell Culture: Protocols for the Preparation and Functional Analysis of Highly Reproducible, Large, Uniform Spheroids and Organoids

Krzysztof Wrzesinski, Helle Sedighi Frandsen, Carlemi Calitz, Chrisna Gouws, Barbara Korzeniowska, and Stephen J. Fey

3. Protocol to Study the Role of Extracellular Vesicles during Induced Stem Cell Differentiation

Kelly C.S. Roballo, Carlos E. Ambrosio, and Juliano C. da Silveira

4. A Simplified Method for Three-Dimensional (3D) Porcine Preantral Follicles Culture Utilizing Hydrophobic Microbioreactors

Malgorzata Duda, Lucia Gizler, and Gabriela Gorczyca

5. Use of Transparent Liquid Marble: Microbioreactor to Culture Cardiospheres

6. Isolation, Culture, and Characterization of Primary Bovine Endometrial, Epithelial, and Stromal Cells for 3D In Vitro Tissue Models

Antonio Murillo and Marta Muñoz

7. Using Decellularization/Recellularization Processes to Prepare Liver and Cardiac Engineered Tissues

Matteo Ghiringhelli, Yousef Abboud, Snizhanna V. Chorna, Irit Huber, Gil Arbel, Amira Gepstein, Georgia Pennarossa, Tiziana A.L. Brevini, and Lior Gepstein

8. Use of Virus-Mimicking Nanoparticles to Investigate Early Infection Events in Upper Airway 3D Models

Georgia Pennarossa, Alireza Fazeli, Sergio Ledda, Fulvio Gandolfi, and Tiziana A.L. Brevini

9. Creation of a Bioengineered Ovary: Isolation of Female Germline Stem Cells for the Repopulation of a Decellularized Ovarian Bio-Scaffold

Georgia Pennarossa, Matteo Ghiringhelli, Fulvio Gandolfi, and Tiziana A.L. Brevini

10. A Two-Step Protocol to Erase Human Skin Fibroblasts and Convert Them into Trophoblast-Like Cells

Sharon Arcuri, Fulvio Gandolfi, Edgardo Somigliana, and Tiziana A.L. Brevini

11. 3D-ViaFlow: A Quantitative Viability Assay for Multicellular Spheroids

Joel Mario Vej-Nielsen and Adelina Rogowska-Wrzesinska

12. Method to Disassemble Spheroids into Core and Rim for Downstream Applications Such as Flow Cytometry, Comet Assay, Transcriptomics, Proteomics, and Lipidomics

Helle Sedighi Frandsen, Martina Stampar, Joel Mario Vej-Nielsen, Bojana Zegura, and Adelina Rogowska-Wrzesinska

13. Isolation of Extracellular Vesicles (EVs) Using Size Exclusion-High Performance Liquid Chromatography (SEC-HPLC)

Keerthie Dissan