Part I: Basic Techniques and Their Applications

1. Physcomitrium patens Protoplasting and Protoplast Transfection

Florence Charlot, Guillaume Goudounet, Fabien Nogué, and Pierre-François Perroud

2. Protoplast Isolation and Transfection in the Single-Cell C₄ Species Bienertia sinuspersici

Shiu-Cheung Lung, Nilanth Yogadasan, Makoto Yanagisawa, and Simon D.X. Chuong

3. Protoplast for Gene Functional Analysis in Arabidopsis

Yanlin Liu and Yan Xiong

Fu-Hui Wu, Yu-Hsuan Yuan, Chen-Tran Hsu, Qiao-Wei Cheng, and Choun-Sea Lin

5. CRISPR DNA- and RNP-Mediated Genome Editing via Nicotiana benthamiana Protoplast Transformation and Regeneration

Raviraj Banakar, Krishan M. Rai, and Feng Zhang

6. Protoplast Isolation and Transfection in Rice

Zhaohui Zhong and Yong Zhang

7. Protoplast Isolation and Transfection in Maize

Monique R. Coy, Shane E. Abbitt, and Mary J. Frank

8. Isolation and Transfection of Maize Endosperm Protoplasts

Yufeng Hu and Yubi Huang

James W. Satterlee and Michael J. Scanlon

10. Protoplast Isolation and Transfection in Wheat

Guangbin Luo, Boshu Li, and Caixia Gao

11. Protoplast Isolation, Transformation, and Regeneration for Forage and Turf Grasses

Zeng-Yu Wang and German Spangenberg

12. Efficient Protoplast Isolation and DNA Transfection for Winter Oilseed Crops, Pennycress (Thlaspi arvense) and Camelina (Camelina sativa)

Minjeong Kang, Keunsub Lee, and Kan Wang

13. Protoplast Isolation, Transfection, and Gene Editing for Soybean (Glycine max)

Gunvant B. Patil, Robert M. Stupar, and Feng Zhang

14. Protoplast Isolation and Transformation in Oil Palm

Mat Yunus Abdul Masani, Ghulam Kadir Ahmad Parveez, Gundula Noll, MD Piji Mohd Al Akmarul Fizree, Ravigadevi Sambanthamurthi, and Dirk Pruefer

Part II: Advanced Systems for High Throughput Analysis and Automation

15. Protoplast Preparation and Fluorescence-Activated Cell Sorting for the Evaluation of Targeted Mutagenesis in Plant Cells

Ward Decaestecker, Norbert Bollier, Rafael A. Buono, Moritz K. Nowack, and Thomas B. Jacobs

16. Characterization of Programmable Transcription Activators in the Model Monocot Setaria viridis via Prot