Preface...
Table of Contents...
Contributing Authors...

1. 1. A New Method to Predict Ion Effects in RNA Folding Li-Zhen Sun and Shi-Jie Chen

2. Computational Generation of RNA Nanorings
Rishabh Sharan, Eckart Bindewald, Wojciech Kasprzak, and Bruce A. Shapiro

3. Protocols for Molecular Dynamics Simulations of RNA Nanostructures

Taejin Kim, Wojciech K. Kasprzak, and Bruce A. Shapiro

4. Rolling Circle Transcription for the Self-Assembly of Multimeric RNAi Structures and its Applications in Nanomedicine
Mihue Jang and Hyung Jun Ahn

5. Computational Prediction of the Immunomodulatory Potential of RNA Sequences
Gandharva Nagpal, Kumardeep Chaudhary, Sandeep Kumar Dhanda, and Gajendra Pal Singh Raghava

6. Co-Transcriptional Production of Chemically Modified RNA Nanoparticles

Maria L. Kireeva, Kirill A. Afonin, Bruce A. Shapiro, and Mikhail Kashlev

7. Supported Fluid Lipid Bilayer as a Scaffold to Direct Assembly of RNA Nanostructures
Aleksandra P. Dabkowska, Agnes Michanek, Luc Jaeger, Arkadiusz Chworos, Tommy Nylandera and Emma Sparr

8. Evaluation of Thermal Stability of RNA Nanoparticles by Temperature Gradient Gel Electrophoresis (TGGE) in Native Condition
Kheiria Benkato, Ben O'Brien, My N. Bui, Daniel Jasinski, Peixuan Guo, and Emil F. Khisamutdinov

9. Design and Crystallography of Self-Assembling RNA Nanostructures
Mark A. Boerneke and Thomas Hermann

10. X-Aptamer Selection and Validation
Ganesh L. Lokesh, Hongyu Wang, Curtis H. Lam, Varatharasa Thiviyanathan, Nancy Ward, David G. Gorenstein and David E. Volk

11. Design and Preparation of Aptamer-siRNA Chimeras (AsiCs) for Targeted Cancer Therapy

Sven Kruspe and Paloma H. Giangrande

12. Cellular Delivery of siRNAs using Bolaamphiphiles
Kshitij Gupta

13. Preparation and Optimization of Lipid-Like Nanoparticles for mRNA Delivery
Bin Li and Yizhou Dong

14. Chitosan Nanoparticles for miRNA Delivery
Merve Denizli, Burcu Aslan, Lingegowda S. Mangala, Dahai Jiang, Cristian Rodriguez-Aguayo, Gabriel Lopez-Berestein, and Anil K. Sood

15. Synthesis of PLGA/Lipid Hybrid Nanoparticles for siRNA Delivery using the Emulsion Method PLGA-PEG/Lipid Nanoparticles for siRNA Delivery

Lei Wang, Benjamin Griffel, and Xiaoyang Xu

16. Oxime Ether Lipids as Transfection Agents: Assembly and Complexation with siRNA
Anu Puri, Serena Zampino, Mathias Viard, and Bruce A. Shapiro

17. Polycationic Probe-Guided Nanopore Single-Molecule Counter for Selective miRNA Detection

Kai Tian, Ruicheng Shi, and Li-Qun Gu

18. Intracellular Re-Association of RNA-DNA Hybrids that Activates RNAi in HIV-Infected Cells
Angelica N. Martins, Weina Ke, Vaishnavi Jawahar, Morriah Striplin, Caryn Striplin, Eric O. Freed, And Kirill A. Afonin

19. Construction and In Vivo Testing of Prokaryotic Riboregulators

Alexander A. Green

20. Preparation of a Conditional RNA Switch

Paul Zakrevsky, Lorena Parlea, Eckart Bindewald, Kirill A. Afonin, and Bruce A. Shapiro

21. Rational Engineering of a Modular Group I Ribozyme to Control its Activity by Self-Dimerization

Takahiro Tanaka, Yoshiya Ikawa, and Shigeyoshi Matsumura

22. CRISPR-Cas RNA Scaffolds for Transcriptional Programming in Yeast

Jesse G. Zalatan

23. Using Planar Phi29 pRNA Three-Way Junction to Control Size and Shape of RNA Nanoparticles for Biodistribution Profiling in Mice

Farzin Haque, Congcong Xu, Daniel L. Jasinski, Hui Li, and Peixuan Guo