Part I: Protein Production

1. High-Throughput Protein Engineering by Massively Parallel Combinatorial Mutagenesis

Yuk Kei Wan, Gigi C.G. Choi, and Alan S.L. Wong

2. Rational Design and Construction of Active-Site Labeled Enzymes

Man-Wah Tsang, Yun-Chung Leung, and Kwok-Yin Wong

3. Screening and Production of Recombinant Human Proteins: Ligation Independent Cloning

Claire Strain-Damerell, Pravin Mahajan, Alejandra Fernandez-Cid, Opher Gileadi, and Nicola Burgess-Brown

4. Screening and Production of Recombinant Human Proteins: Protein Production in E. coli

Nicola A. Burgess-Brown, Pravin Mahajan, Claire Strain-Damerell, Alejandra Fernandez-Cid, Opher Gileadi, and Susanne Gräslund

5. Screening and Production of Recombinant Human Proteins: Protein Production in Insect Cells

Pravin Mahajan, Claire Strain-Damerell, Shubhashish Mukhopadhyay, Alejandra Fernandez-Cid, Opher Gileadi, and Nicola A. Burgess-Brown

6. Expression Screening of Human Integral Membrane Proteins Using BacMam

Pravin Mahajan, Katherine Ellis, Shubhashish Mukhopadhyay, Alejandra Fernandez-Cid, Gamma Chi, Henry Man, Katharina L. Dürr, and Nicola A. Burgess-Brown

7. High-Throughput Expression Screening in Mammalian Suspension Cells

Susan D. Chapple and Michael R. Dyson

8. In Vitro Production of Perdeuterated Proteins in H₂O for Biomolecular NMR Studies

Lionel Imbert, Rachel Lenoir-Capello, Elodie Crublet, Alicia Vallet, Rida Awad, Isabel Ayala, Celine Juillan-Binard, Hubert Mayerhofer, Rime Kerfah, Pierre Gans, Emeric Miclet, and Jerome Boisbouvier

9. Minimizing Heterogeneity of Protein Samples for Metal Transporter Proteins Using SAXS and Metal Radioisotopes

Shah Kamranur Rahman

Part II: Structural Analyses and Data Management

10. Hydrogen/Deuterium Exchange-Mass Spectrometry for Probing Changes in Conformation and Dynamics of Proteins

Pui-Kin So

András Micsonai, Éva Bulyáki, and József Kardos

12. Navigating the Global Protein-Protein Interaction Landscape Using iRefWeb

Andrei L. Turinsky, Sam Dupont, Alexander Botzki, Sabry Razick, Brian Turner,

Ian M. Donaldson, and Shoshana J. Wodak

13. State-of-the-Art Data Management: Improving the Reproducibility, Consistency, and Traceability of Structural Biology and In Vitro Biochemical Experiments

David Cooper, Marek Grabowski, Matthew D. Zimmerman, Przemyslaw J. Porebski, Ivan G. Shabalin, Magdalena Woinska, Marcin J. Domagalski, Heping Zheng, Piotr Sroka, Marcin Cymborowski, Mateusz P. Czub, Ewa Niedzial