This volume includes a series of
protocols focused on mitotic spindle assembly and function. The methods covered
in this book feature a broad range of techniques from basic microscopy to the
study of spindle physiologies relevant to cancer. These methods can be applied
to diverse model systems that range from the cell-free Xenopus egg
extract system to the moss Physcomitrella patens, in an effort to
demonstrate the key contributions made by researchers using multiple model
organisms. Chapters in The Mitotic Spindle: Methods and Protocols integrate cutting-edge technologies that have only become available due to the
cross-disciplinary efforts, such as ATP analogue sensitive inhibition of
mitotic kinases. Written in the highly successful Methods in Molecular Biology
series format, chapters include introductions to their respective topics,
lists of the necessary materials and reagents, step-by-step, readily
reproducible laboratory protocols, and tips on troubleshooting and avoiding
known pitfalls.





Thorough and informative, The
Mitotic Spindle: Methods and Protocols, is a valuable resource for
researchers who are new to mitosis or are already experts in the field.