This volume discusses the latest advancements in the field of antigen processing and presentation, and the complex assays and techniques that have been devised to help pick apart molecular events along this pathway. The chapters in this book cover topics such as how to make and analyze MHC molecules and their cargo; analyzing the function chaperones that regulate antigen presentation; enzymes that generate antigenic peptides; how to make and analyze T cell receptors; and presenting a series of functional assays to follow adaptive immune responses to antigen presentation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Antigen Processing and Presentation: Methods and Protocols is a valuable resource for all researchers who are looking to carry the field forward, validate key findings, and help newcomers make meaningful contributions.