This book constitutes the refereed proceedings of the 16th International Conference on Cellular Automata for Research and Industry, Cellular Automata, ACRI 2024, held in Florence, Italy, in September 9–11, 2024.

The 20 full papers presented were carefully reviewed and selected from 33 submissions. They were organized in the following topical sections: theory, mathematical and physical models; computational aspects and applications; social and biological models.