The discovery of CRISPR has revolutionized crop improvement in agriculture and is emerging as a key factor in addressing the global food security challenges. This new volume highlights the new frontiers and applications of the cutting-edge CRISPR-based gene editing systems and discusses how to harness the power of these tools for improving food crops and developing climate-smart crops to meet the global food demand in the future. The volume reviews the applications of CRISPR-Cas systems in improving plants’ biotic and abiotic stress tolerance, increasing nutritional quality and yield, and more. The book also reviews the application of CRISPR-Cas in the development of desirable livestock and large animals to achieve global food security. In addition, the regulatory aspects and the risks associated with this technology are discussed as well.