This book constitutes the refereed proceedings of the 14th International Conference on Games and Learning Alliance, GALA 2025, held in Utrecht, The Netherlands during November 19–21, 2025.

The 33 full papers and 24 short papers included in this book were carefully reviewed and selected from 142 submissions. They were organized in topical sections as follows: educational science track; game design track; media and cultural studies track; technology track; application track; and short papers.